Preparation of Newcastle disease virus hemagglutination-inhibition test antigen.


A method of preparing antigen for Newcastle hemagglutination-inhibition (HI) tests is described; polyethylene glycol precipitation is used for the virus concentration. The virus was inactivated with either formalin or beta-propiolactone. Glycerin was used to stabilize the hemagglutinin activity of the antigen. The stability, uninfectivity, and slow eluting… (More)


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