Preparation of Kupffer cell enriched non-parenchymal liver cells with high yield and reduced damage of surface markers by a modified method for flow cytometry.

@article{Xu2013PreparationOK,
  title={Preparation of Kupffer cell enriched non-parenchymal liver cells with high yield and reduced damage of surface markers by a modified method for flow cytometry.},
  author={Fan Xu and Peng Yong Zhen and Yu Zheng and Feng Lijuan and Yang Aiting and Cong Min and You Jin Hong and Jia Jidong},
  journal={Cell biology international},
  year={2013},
  volume={37 4},
  pages={284-91}
}
The aim of this study was to optimise a collagenase perfusion protocol for the isolation of a liver non-parenchymal cell (NPC) suspension enriched for Kupffer cells that reduced damage to F4/80 antigen cell surface expression to allow analysis by flow cytometry. Kupffer cell-enriched liver NPCs were isolated from C57BL/6 mice using different protocols. Flow… CONTINUE READING