Preparation of DNA from Embryonic Stem Cells or Other Cultured Cells.

Abstract

Characterization of embryonic stem (ES) cell-mediated genome alterations, including random insertional transgenesis, gene trapping, gene targeting, and site-specific recombinase-mediated changes, is performed mostly at the ES cell level, before the introduction of these alterations into a mouse. A detailed characterization requires a larger amount of DNA… (More)
DOI: 10.1101/pdb.prot092718

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