Preparation of DNA and protein micro arrays on glass slides coated with an agarose film.

@article{Afanassiev2000PreparationOD,
  title={Preparation of DNA and protein micro arrays on glass slides coated with an agarose film.},
  author={V Afanassiev and V Hanemann and Stefan W{\"o}lfl},
  journal={Nucleic acids research},
  year={2000},
  volume={28 12},
  pages={E66}
}
A thin layered agarose film on microscope slides provides a versatile support for the preparation of arrayed molecular libraries. An activation step leading to the formation of aldehyde groups in the agarose creates reactive sites that allow covalent immobilization of molecules containing amino groups. Arrays of oligonucleotides and PCR products were prepared by tip printing. After hybridization with complementary fluorescence labeled nucleic acid probes strong fluorescence signals of sequence… CONTINUE READING
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Handbook of Fluorescent Probes and Research Chemicals, Sixth Edition

  • V. Afanassiev, J. Troppmair, M. Schuler, Weber, Ch, U. R. Rapp
  • Biochim. Biophys. Acta,
  • 1998

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