Preparation of Concanavalin A-β-galactosidase conjugate and its application in lactose hydrolysis

@article{Khare2008PreparationOC,
  title={Preparation of Concanavalin A-β-galactosidase conjugate and its application in lactose hydrolysis},
  author={Sunil Kumar Khare and Munishwar Nath Gupta},
  journal={Journal of Biosciences},
  year={2008},
  volume={13},
  pages={47-54}
}
A Concanavalin A-β-galactosidase conjugate was prepared using glutaraldehyde as the crosslmking reagent. The conjugate bound to Sephadex G-50 beads was more thermostable and hydrolyzed lactose faster than the free enzyme. The immobilized enzyme may prove useful in the preparation of low lactose milk which is required by persons suffering from lactose… CONTINUE READING