Preparation of 5- and 6-(aminomethyl)fluorescein.

  title={Preparation of 5- and 6-(aminomethyl)fluorescein.},
  author={Philip G. Mattingly},
  journal={Bioconjugate chemistry},
  volume={3 5},
5(6)-Carboxyfluorescein is protected as the diacetate then reduced to 5(6)-(hydroxymethyl)fluorescein diacetate. The separated isomers are subjected to a Mitsunobu reaction with dibenzyl imidodicarbonate, yielding diprotected 5- and 6-(aminomethyl)fluorescein diacetate. Methanolysis of the acetates followed by deprotection with HBr/acetic acid gives 5- and 6-(aminomethyl)fluorescein hydrobromide. 
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