Preparation of 5′-GMP-rich yeast extracts from spent brewer's yeast

@article{Sombutyanuchit2001PreparationO5,
  title={Preparation of 5′-GMP-rich yeast extracts from spent brewer's yeast},
  author={P. Sombutyanuchit and Manop Suphantharika and Cornelis Verduyn},
  journal={World Journal of Microbiology and Biotechnology},
  year={2001},
  volume={17},
  pages={163-168}
}
Spent brewer's yeast was autolysed and used as a raw material for the preparation of 5′-GMP-rich yeast extracts. Malt rootlets were used as a source of 5′-phosphodiesterase. The crude enzyme was extracted from malt rootlets and pretreated to inactivate 5′-nucleotidase. The optimum pretreatment conditions were heating at 65 °C for 30 min or 70 °C for 7 min. The effects of autolysis time, phosphodiesterase concentration and incubation period on 5′-GMP content were examined. The suitable autolysis… 

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