Preparation, characterization, and in vitro cytotoxicity study of cationic PCL-pluronic-PCL (PCFC) nanoparticles for gene delivery.

Abstract

In this article, poly(epsilon-caprolactone)-pluronic-poly(epsilon-caprolactone) (PCFC) copolymer was synthesized by ring-opening polymerization and characterized by 1H-NMR and GPC. Cationic PCFC nanoparticles were prepared at one-step by modified emulsion solvent evaporation method using cetyltrimethylammonium bromide as the stabilizer. The cytotoxicity of PCFC nanoparticles was studied here with and without serum. The obtained cationic PCFC nanoparticles were employed to condense and adsorb DNA onto its surface. And it could protect plasmid GFP (pGFP) from enzymatic degradation and acidic degradation in a certain period. Release behavior of pGFP from the pGFP/PCFC nanoparticles complex was also studied in vitro. The obtained cationic PCEC nanoparticles might have great potential application in DNA delivery.

DOI: 10.1002/jbm.a.31950

Cite this paper

@article{Zhao2009PreparationCA, title={Preparation, characterization, and in vitro cytotoxicity study of cationic PCL-pluronic-PCL (PCFC) nanoparticles for gene delivery.}, author={Jian Zhao and Maling Gou and Mei Dai and Xingyi Li and Mei Ai Cao and Meijuan Huang and Yanjun Wen and Bing Kan and Zhiyong Qian and Yu-Quan Wei}, journal={Journal of biomedical materials research. Part A}, year={2009}, volume={90 2}, pages={506-13} }