Preparation and use of specific antibodies to the beta-I and beta-II subunits of gonadotropic hormone from Fundulus heteroclitus pituitary.

@article{Calman2001PreparationAU,
  title={Preparation and use of specific antibodies to the beta-I and beta-II subunits of gonadotropic hormone from Fundulus heteroclitus pituitary.},
  author={B G Calman and Y W Lin and Robin A. Wallace},
  journal={General and comparative endocrinology},
  year={2001},
  volume={123 2},
  pages={203-9}
}
Fundulus heteroclitus naturally spawns with a semilunar periodicity throughout most of the year in its southernmost habitat, an activity that can be maintained in the laboratory. The alpha and two beta subunits comprising F. heteroclitus gonadotropic hormones (GtHs) I and II have been sequenced, and antibodies have been raised against unique peptides found in each of the two beta subunits. On immunoblots of pituitary proteins, each antibody recognizes a single band with a molecular mass of 16… CONTINUE READING

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