Preparation and testing of stationary phases and modified capillaries for affinity chromatography and affinity capillary electrophoresis of pepsin.

@article{Varilov2005PreparationAT,
  title={Preparation and testing of stationary phases and modified capillaries for affinity chromatography and affinity capillary electrophoresis of pepsin.},
  author={Tereza Varilov{\'a} and Alice Vr{\'a}nkov{\'a} and Vĕra Pac{\'a}kov{\'a} and Marie Tich{\'a} and Karel Stul{\'i}k},
  journal={Journal of chromatography. A},
  year={2005},
  volume={1084 1-2},
  pages={207-13}
}
Three stationary phases have been prepared for affinity liquid chromatography isolation and separation of porcine and human pepsin. The phases contain 3,5-diiodo-L-tyrosine (DIT) bound to the supports HEMA BIO VS, HEMA BIO E and EPOXY TOYOPEARL. These phases have been tested on a model sample of porcine pepsin A and applied to human pepsin. Fractions have been collected and the chymase activity determined in selected analyses. For affinity CE, capillaries have been prepared by modifying the… CONTINUE READING