Preparation and testing of goat anti--thymocyte globulin.

  title={Preparation and testing of goat anti--thymocyte globulin.},
  author={Dana Christopher Mears and A. G. R. Sheil and G. E. Kelly and P C Beerenfels},
  journal={The Australian journal of experimental biology and medical science},
  volume={54 5},
This study details methods of production, purification and testing of large volumes of uniformly potent, non-toxic, anti-human thymocyte globulin (ATG). Plasma from 70 goats immunised with human thymic lymphocytes was fractionated by ammonium sulphate precipitation to its IgG component. Contaminating antibodies were removed by absorption methods. After fractionation and absorption, ATG retained its ability to inhibit the formation of rosettes by human lymphocytes with sheep red blood cells… CONTINUE READING