Preparation and regeneration of spheroplasts from Arthrospira platensis (Spirulina)

@article{Gan2004PreparationAR,
  title={Preparation and regeneration of spheroplasts from Arthrospira platensis (Spirulina)},
  author={Xuhua Gan and Xinyun Tang and Chengying Shi and Benfan Wang and Yuanyuan Cao and Liangxia Zhao},
  journal={Journal of Applied Phycology},
  year={2004},
  volume={16},
  pages={513-517}
}
Ultrasonic pretreatment, lysozyme, inorganic osmotics and bovine albumin were used to prepare the spheroplasts of Arthrospira platensis (Spirulina platensis). The average cell number of the fragments from the filaments of strain A9 was about 2.2 cells after 80-s ultrasonic pretreatment. These fragments could regenerate and were suitable material for isolating spheroplasts, so the optimum conditions for doing this were investigated. The best enzymolysis parameters were designed. During the… CONTINUE READING

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