Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha.

@article{Tanaka1982PreparationAP,
  title={Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha.},
  author={Shinya Tanaka and Sen Zhe Hu and Teresa S-F Wang and Daniel S. Korn},
  journal={The Journal of biological chemistry},
  year={1982},
  volume={257 14},
  pages={8386-90}
}
We report the successful establishment of 16 stable murine hybridoma monoclones that produce homogeneous antibodies against KB cell DNA polymerase alpha. All of the antibodies exhibit specific binding of polymerase alpha activity, and 3 of them possess anti-polymerase alpha neutralizing activity. None of the antibodies interacts detectably with KB cell DNA polymerases beta or gamma. All of the 5 antibodies so far examined demonstrate linear Scatchard binding plots and very high binding… CONTINUE READING

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