Preparation and physical characterization of a homogeneous population of monomeric nucleosomes from HeLa cells.

@article{Whitlock1976PreparationAP,
  title={Preparation and physical characterization of a homogeneous population of monomeric nucleosomes from HeLa cells.},
  author={J P Whitlock and R. T. Simpson},
  journal={Nucleic acids research},
  year={1976},
  volume={3 9},
  pages={2255-66}
}
  • J P Whitlock, R. T. Simpson
  • Published 1976 in Nucleic acids research
We describe a method of isolating a homogeneous population of "trimmed" monomeric nucleosomes from Hela cells. These nucleoprotein particles contain a 140 +/- 5 base pair length of DNA and have a histone/DNA ratio of 1.2. They lack H1 and contain equal amounts of the four smaller histones. The DNA contains no single strand nicks. The particles sediment with an S20,w of 11S in D2O density gradients. After formaldehyde fixation, they band at a density of 1.4370 in neutral CsCl. Digestion of… CONTINUE READING