Preparation and nuclease activity of hybrid "metallotris(methylpyridinium)porphyrin oligonucleotide" molecules having a 3'-loop for protection against 3'-exonucleases.

@article{Mestre1995PreparationAN,
  title={Preparation and nuclease activity of hybrid "metallotris(methylpyridinium)porphyrin oligonucleotide" molecules having a 3'-loop for protection against 3'-exonucleases.},
  author={B{\'e}atrice Mestre and Genevi{\`e}ve Pratviel and Bernard Meunier},
  journal={Bioconjugate chemistry},
  year={1995},
  volume={6 4},
  pages={466-72}
}
A 5'-GCGAAAGC minihairpin structure was added to the 3'-end of an oligonucleotide substituted at the 5'-end by a manganese cationic porphyrin in order to enhance the 3'-exonuclease resistance of these cleaver-antisense molecules. The influence of this minihairpin on the 3'-exonuclease resistance, the binding affinity to a target ssDNA, and the cleaving… CONTINUE READING