Preparation and maintenance of organotypic slice cultures of CNS tissue.

  title={Preparation and maintenance of organotypic slice cultures of CNS tissue.},
  author={Beat H. G{\"a}hwiler and Scott M. Thompson and Dieter Muller},
  journal={Current protocols in neuroscience},
  volume={Chapter 6},
  pages={Unit 6.11}
Organotypic slice cultures are the in vitro method of choice for applications requiring long-term survival of the preparation and a high degree of cellular differentiation and organization resembling that of the original tissue. Long-term survival is achieved by culturing slices at the air/liquid interface, either by continuously rotating the preparation (roller-tube cultures) or by culturing them on semiporous membranes (stationary interface cultures). Both culture techniques yield nerve cells… CONTINUE READING

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