Preparation and kinetic properties of gel entrapped urate oxidase.

@article{Hinberg1975PreparationAK,
  title={Preparation and kinetic properties of gel entrapped urate oxidase.},
  author={Irwin Hinberg and K O'driscoll},
  journal={Biotechnology and bioengineering},
  year={1975},
  volume={17 10},
  pages={1435-41}
}
Urate oxidase from hog liver (urate: oxygen oxidoreductase, EC 1.7.33) has been entrapped in a crosslinked 2-hydroxyethyl methacrylate gel with a 47% retention of activity. The kinetic behavior of the gel entrapped enzyme has been studied in a slurried tank reactor using uric acid as substrate. Internal diffusion effects were found to be negligible for… CONTINUE READING