Preparation and in vitro characteristics of a blood substitute based on pyridoxylated polyhemoglobin.

Abstract

Stroma-free hemoglobin (SFHb) is prepared from outdated blood. Red cells are washed, lysed in hypotonic phosphate buffer, and stromal lipid is then removed by toluene extraction and high-speed centrifugation. Pyridoxal-phosphate (PP) added in a 4:1 molar ratio to deoxygenated SFHb, is covalently linked across the polyphosphate binding site of the Hb… (More)

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