Preparation and fractionation of conjugated trienes from α-linolenic acid and their growth-inhibitory effects on human tumor cells and fibroblasts

@article{Igarashi2005PreparationAF,
  title={Preparation and fractionation of conjugated trienes from α-linolenic acid and their growth-inhibitory effects on human tumor cells and fibroblasts},
  author={Miki Igarashi and Teruo Miyazawa},
  journal={Lipids},
  year={2005},
  volume={40},
  pages={109-113}
}
Conjugated α-linolenic acid (ClnA) was prepared from α-linolenic acid (9,12,15–18∶3n−3, LnA) by alkaline treatment; we fractionated CLnA into three peaks by reversed-phase column-HPLC as evidenced by monitoring absorbance at 205, 235, and 268 nm. Peak I was a conjugated dienoic FA derived from LnA, whereas Peaks II and III were conjugated trienoic LnA. Proton NMR analysis showed that Peak III consisted of the all-trans isomer. The methylated Peak III was further divided into five peaks (Peaks… CONTINUE READING