Preparation and evaluation of a non-viral gene vector for SiRNA: Multifunctional envelope-type nano device (.).

  • Ying Zhang, Haitian Wei, +5 authors Yong Sun
  • Published 2016 in Artificial cells, nanomedicine, and biotechnology

Abstract

We prepared and evaluated a multifunctional envelope-type nano device (MEND) as a liver-targeting and long-circulation carrier for SiRNA. The polymer GA-PEG-Pp-DOPE was synthesized by modifying polyethylene glycol (PEG) with glycyrrhetinic acid (GA), peptide (Pp), and dioleoyl phosphoethanolamine (DOPE). The Pp is a substrate of matrix metalloproteinase 2. MEND was prepared with GA-PEG-Pp-DOPE and cationic phospholipids by the filming-rehydration method, and the orthogonal test was applied to optimize the prescription. The results of the biological evaluation results suggest that MEND is a promising delivery system for SiRNA.

DOI: 10.3109/21691401.2015.1024840

Cite this paper

@article{Zhang2016PreparationAE, title={Preparation and evaluation of a non-viral gene vector for SiRNA: Multifunctional envelope-type nano device (.).}, author={Ying Zhang and Haitian Wei and Lisa X. Xu and Guowen Yan and Chao Ma and Miao Yu and Chen Wei and Yong Sun}, journal={Artificial cells, nanomedicine, and biotechnology}, year={2016}, volume={44 5}, pages={1259-65} }