Preparation and evaluation of a mixed-bed immunoaffinity column for selective purification of sixteen sulfonamides in pork muscle.

@article{Li2012PreparationAE,
  title={Preparation and evaluation of a mixed-bed immunoaffinity column for selective purification of sixteen sulfonamides in pork muscle.},
  author={Yingguo Li and Yiqiang Chen and Zhengguo Li and Lei Zhang and Xianliang Li and Cunxian Xi and Guo-min Wang and Xiongbiao Wang and Qi Guo and Na Li},
  journal={Journal of chromatographic science},
  year={2012},
  volume={50 3},
  pages={167-74}
}
This paper describes the preparation of a novel mixed-bed immunoaffinity chromatography (IAC) column by coupling four monoclonal antibodies against different sulfonamides (SAs) to Sepharose 4B. The IAC column can be used to simultaneously extract and purify 16 SAs in pork muscle. The dynamic column capacities for all SAs in mixed standard solution were between 312 and 479 ng/mL gel. After simple extraction and IAC cleanup, the sample solution can be directly injected for liquid chromatography… CONTINUE READING

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