Preparation and characterization of monoclonal antibody against protein tyrosine phosphatase PRL-3.

@article{Peng2004PreparationAC,
  title={Preparation and characterization of monoclonal antibody against protein tyrosine phosphatase PRL-3.},
  author={Lirong Peng and Yan Li and Ling Meng and Cheng-chao Shou},
  journal={Hybridoma and hybridomics},
  year={2004},
  volume={23 1},
  pages={23-7}
}
PRL-3 is a newly identified nonclassical protein tyrosine phosphatase associated with colorectal cancer metastasis. To prepare specific monoclonal antibody (MAb) against PRL-3, cDNAs of PRL-1, -2, and -3 were cloned by RT-PCR and inserted into prokaryotic expression vector pGEX-4T1, respectively. The fusion proteins were expressed in Escherichia coli… CONTINUE READING