Preparation and characterization of membrane fractions enriched in outer and inner envelope membranes from spinach chloroplasts. I. Electrophoretic and immunochemical analyses.

@article{Block1983PreparationAC,
  title={Preparation and characterization of membrane fractions enriched in outer and inner envelope membranes from spinach chloroplasts. I. Electrophoretic and immunochemical analyses.},
  author={Maryse A Block and A.- J. Dorne and Jacques Joyard and Roland Douce},
  journal={The Journal of biological chemistry},
  year={1983},
  volume={258 21},
  pages={13273-80}
}
We have developed a fast and reliable method for the separation of two membrane fractions respectively enriched in outer and inner envelope membranes from isolated, intact, purified spinach chloroplasts kept in a hypertonic medium (0.6 M mannitol). This separation was achieved by osmotically shrinking the inner envelope membrane, thus widening the… CONTINUE READING