Preparation and characterization of dog pancreas microsomal membranes specifically depleted of protein disulphide-isomerase.

@article{Paver1989PreparationAC,
  title={Preparation and characterization of dog pancreas microsomal membranes specifically depleted of protein disulphide-isomerase.},
  author={J L Paver and H C Hawkins and R. B. Freedman},
  journal={The Biochemical journal},
  year={1989},
  volume={257 3},
  pages={657-63}
}
1. The selective release of protein disulphide-isomerase from dog pancreas and rat liver microsomal membranes was studied to throw light on the mechanisms of retention of this enzyme within the endoplasmic reticulum, and in order to prepare microsomal membranes specifically depleted of the enzyme. 2. Protein disulphide-isomerase was quantitatively released from dog pancreas microsomal membranes by washing at pH 9 and above, as demonstrated both by enzyme assay and by immunoblotting analysis. 3… CONTINUE READING

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