Preparation and characterization of interleukin-2-gelonin conjugates made using different cross-linking reagents.

@article{McIntyre1994PreparationAC,
  title={Preparation and characterization of interleukin-2-gelonin conjugates made using different cross-linking reagents.},
  author={Gregory McIntyre and Charles F. Scott and Jerome Ritz and Walter A. Bl{\"a}ttler and J. G. Lambert},
  journal={Bioconjugate chemistry},
  year={1994},
  volume={5 1},
  pages={88-97}
}
Conjugates of IL-2 with the ribosome-inactivating protein gelonin were prepared using heterobifunctional reagents to link the proteins via disulfide, acid-labile, and noncleavable linkers. In each case, one protein was modified using 2-iminothiolane. The sulfhydryl groups so introduced were then reacted either with 2-nitro-5-dithiobenzoate groups or with iodoacetamido groups which had been introduced into the second protein. In the case of the acid-labile linkage, a reagent which forms a labile… CONTINUE READING