Preparation and characterization of DNA aptamer based spin column for enrichment and separation of histones.

Abstract

Core histones play a critical role in the regulation of chromatin-templated biological process. Here we have developed a novel approach to enrich histone proteins based on the recognition of a specific aptamer. The method shows good selectivity toward histone proteins and is able to extract histones directly from complex whole cell lysates.

DOI: 10.1039/c2cc32376a

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Cite this paper

@article{Shao2012PreparationAC, title={Preparation and characterization of DNA aptamer based spin column for enrichment and separation of histones.}, author={Nan Shao and Kai Zhang and Ying Chen and Xiwen He and Yukui Zhang}, journal={Chemical communications}, year={2012}, volume={48 53}, pages={6684-6} }