Preparation and application of monoclonal antibodies against oxidized DJ-1. Significant elevation of oxidized DJ-1 in erythrocytes of early-stage Parkinson disease patients.

@article{Saito2009PreparationAA,
  title={Preparation and application of monoclonal antibodies against oxidized DJ-1. Significant elevation of oxidized DJ-1 in erythrocytes of early-stage Parkinson disease patients.},
  author={Yoshiro Saito and Takao Hamakubo and Yasukazu Yoshida and Yoko Ogawa and Yasuo Hara and Harutoshi Fujimura and Yasuharu Imai and Hiroko Iwanari and Yasuhiro Mochizuki and Mototada Shichiri and Keiko Nishio and Tomoya Kinumi and Noriko Noguchi and Tatsuhiko Kodama and Etsuo Niki},
  journal={Neuroscience letters},
  year={2009},
  volume={465 1},
  pages={1-5}
}
DJ-1 was initially identified as a novel oncogene and has recently been found to be a causative gene for a familial form of Parkinson's disease (PD), viz, PARK7. Cysteine residue at position 106 (Cys-106) in DJ-1 was found to be oxidized preferentially under oxidative stress. In the present study, we developed specific antibodies against Cys-106-oxidized DJ-1 using baculovirus particles displaying the surface glycoprotein gp64-fusion protein as the immunizing agent. Western blot analysis… CONTINUE READING

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