Preparation and Characterization of Membrane Fractions Enriched Outer and Inner Envelope Membranes from Spinach Chloroplasts

@inproceedings{Block2001PreparationAC,
  title={Preparation and Characterization of Membrane Fractions Enriched Outer and Inner Envelope Membranes from Spinach Chloroplasts},
  author={Maryse A Block and Albert-Jean Dorne and Jacques Joyard and Roland Douce},
  year={2001}
}
We have developed a fast and reliable method for the separation of two membrane fractions respectively enriched in outer and inner envelope membranes from isolated, intact, purified spinach chloroplasts kept in a hypertonic medium (0.6 M mannitol). This separation was achieved by osmotically shrinking the inner envelope membrane, thus widening the… CONTINUE READING