Preparation, culture, and immortalization of mouse embryonic fibroblasts.

@article{Xu2005PreparationCA,
  title={Preparation, culture, and immortalization of mouse embryonic fibroblasts.},
  author={Jianming Xu},
  journal={Current protocols in molecular biology},
  year={2005},
  volume={Chapter 28},
  pages={Unit 28.1}
}
Genetically manipulated transgenic and gene-targeted mouse models are indispensible tools used in defining the role of genes in development and organ function. Similarly, cells isolated from these mice bear the same genetic alterations and therefore can be extremely useful for studying the molecular and cellular mechanisms and regulatory gene networks… CONTINUE READING