Preparation, Sensitivity, andSpecificity of Limulus Lysate forEndotoxin Assay

@inproceedings{Smith1973PreparationSA,
  title={Preparation, Sensitivity, andSpecificity of Limulus Lysate forEndotoxin Assay},
  author={Fred K. Smith},
  year={1973}
}
Limulus amoebocyte lysate was prepared fromatotal of180crabs during 1971 and1972byusing a slightly modified lysate preparation procedure. Marked variability oflysate potency was notedbothyears.Inaddition, lysate quality appeared diminished in1972as compared with1971. Different lysate batches were evaluated forpotencybyusing a variety ofendotoxin preparations. Variations inbatchpotencies were observed, butlittle variation inreactivity among different endotoxin preparations was noted… CONTINUE READING