Premature chromosome condensation and cell cycle analysis.

@article{Rao1977PrematureCC,
  title={Premature chromosome condensation and cell cycle analysis.},
  author={P. Nagesh Rao and Brenda A. Wilson and Theodore T. Puck},
  journal={Journal of cellular physiology},
  year={1977},
  volume={91 1},
  pages={131-41}
}
The application of the phenomenon of premature chromosome condensation for cell cycle analysis in HeLa and CHO cells has been examined. Random populations of HeLa and CHO cells pulse labelled with H3-TdR were separately fused with mitotic HeLa cells using U.V. inactivated Sendai virus. The resulting prematurely condensed chromosomes (PCC) were scored and classified into G1, S and G2-PCC on the basis of both morphological and autoradiographic data, The results of this study indicated that the G1… CONTINUE READING
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