Prelysosomal convergence of autophagic and endocytic pathways.

  title={Prelysosomal convergence of autophagic and endocytic pathways.},
  author={P B Gordon and Per O. Seglen},
  journal={Biochemical and biophysical research communications},
  volume={151 1},
[14C]Lactose, electroinjected into the cytosol of isolated rat hepatocytes, was sequestered by autophagy, transferred to lysosomes and eventually hydrolysed. Asparagine prevented the fusion between prelysosomal autophagic vacuoles and lysosomes, and caused lactose to accumulate in the former. However, if the hepatocytes were simultaneously allowed to endocytose added beta-galactosidase, no lactose accumulation occurred. These results suggest that autophagically sequestered lactose and… CONTINUE READING