Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillance

@inproceedings{Sylvestre2013PreliminaryEO,
  title={Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillance},
  author={Gabriel Sylvestre and Mariana Gandini and Jos{\'e}lio Maria Galv{\~a}o de Ara{\'u}jo and Claire Fernandes Kubelka and Ricardo Lourenço-de-Oliveira and Rafael Maciel-de-Freitas},
  booktitle={Parasites & Vectors},
  year={2013}
}
Surveillance is a critical component of any dengue prevention and control programme. Herein, we investigate the efficiency of the commercial kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus (DENV) antigens in Aedes aegypti mosquitoes infected under laboratory conditions. Under insectary conditions, four to five day-old mosquitoes were orally challenged with DENV-2 titer of 3.6 x 105 PFU equivalent/ml, incubated for 14 days and then killed. At ten time-points following mosquito death (0… CONTINUE READING
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