Preliminary evaluation of one conventional nested and two real-time PCR assays for the detection of Toxoplasma gondii in immunocompromised patients.

@article{Hierl2004PreliminaryEO,
  title={Preliminary evaluation of one conventional nested and two real-time PCR assays for the detection of Toxoplasma gondii in immunocompromised patients.},
  author={Thomas Hierl and Udo Reischl and Peter Lang and Holger F. Hebart and Maik Stark and Pierre Kyme and I. Autenrieth},
  journal={Journal of medical microbiology},
  year={2004},
  volume={53 Pt 7},
  pages={629-32}
}
Toxoplasma reactivation is a serious complication in patients receiving allogenic stem cell transplantation. Real-time PCR assays allow a rapid diagnosis of toxoplasma infection; however, no comparative data are available on the performance of real-time PCR protocols under routine conditions. Therefore, the aim of this study was to amplify Toxoplasma gondii DNA from routine samples of allogenic stem cell recipients using two real-time PCR assays on a LightCycler, and using conventional nested… CONTINUE READING

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