Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes.

Abstract

Ureases from both jack bean (Canavalia ensiformis) seeds and Klebsiella aerogenes have been crystallized by the hanging drop method. The plant-derived urease crystals are regular octahedra analogous to those obtained by Sumner. Preliminary X-ray diffraction studies show that the crystals belong to the cubic space group F4(1)32, with a = 364 A, and appear to contain one or two subunits in the asymmetric unit. Using a synchrotron source, the crystals diffract to near 3.5 A resolution. Crystals of urease from K. aerogenes belong to the cubic space group I23 or I2(1)3, with a = 170.8 A and appear to contain a single catalytic unit per asymmetric unit. The crystals diffract to better than 2.0 A resolution and are well suited for structural analysis.

Cite this paper

@article{Jabri1992PreliminaryCS, title={Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes.}, author={Evelyn Jabri and Moses Lee and Robert P. Hausinger and P. Andrew Karplus}, journal={Journal of molecular biology}, year={1992}, volume={227 3}, pages={934-7} }