Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood.

@article{Wallet2010PreliminaryCS,
  title={Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood.},
  author={Fr{\'e}d{\'e}ric Wallet and Saad Nseir and Louis Baumann and St{\'e}phanie Herwegh and B Sendid and Marie Boulo and Micheline Roussel-Delvallez and A. V. Durocher and Ren{\'e} J. Courcol},
  journal={Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases},
  year={2010},
  volume={16 6},
  pages={774-9}
}
Early diagnosis of sepsis, rapid identification of the causative pathogen(s) and prompt initiation of appropriate antibiotic treatment have a combined impact on mortality due to sepsis. In this observational study, a new DNA-based system (LightCycler SeptiFast (LC-SF) test; Roche Diagnostics) allowing detection of 16 pathogens at the species level and four groups of pathogens at the genus level has been evaluated and compared with conventional blood cultures (BCs). One hundred BC and LC-SF… CONTINUE READING

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