Preliminary X-ray diffraction analysis of YcdB from Escherichia coli: a novel haem-containing and Tat-­secreted periplasmic protein with a potential role in iron transport

Abstract

YcdB is a periplasmic haem-containing protein from Escherichia coli that has a potential role in iron transport. It is currently the only reported haem-containing Tat-secreted substrate. Here, the overexpression, purification, crystallization and structure determination at 2.0 A resolution are reported for the apo form of the protein. The apo-YcdB structure resembles those of members of the haem-dependent peroxidase family and thus confirms that YcdB is also a member of this family. Haem-soaking experiments with preformed apo-YcdB crystals have been optimized to successfully generate haem-containing YcdB crystals that diffract to 2.9 A. Completion of model building and structure refinement are under way.

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@article{Cartron2007PreliminaryXD, title={Preliminary X-ray diffraction analysis of YcdB from Escherichia coli: a novel haem-containing and Tat-­secreted periplasmic protein with a potential role in iron transport}, author={Micha{\"{e}l L. Cartron and Sue A. Mitchell and Mark R. Woodhall and Simon C. Andrews and Kimberly A. Watson}, journal={Acta Crystallographica Section F: Structural Biology and Crystallization Communications}, year={2007}, volume={63}, pages={37 - 41} }