Preliminary Observations on Responses of Embryonic and Larval Pacific Herring, Clupea pallasi, to Neutral Fraction Biodegradation Products of Weathered Alaska North Slope Oil

  title={Preliminary Observations on Responses of Embryonic and Larval Pacific Herring, Clupea pallasi, to Neutral Fraction Biodegradation Products of Weathered Alaska North Slope Oil},
  author={Douglas P. Middaugh and M. E. Shelton and Jr. C. L. McKenney and Gary N. Cherr and Peter J. Chapman and Lee A. Courtney},
  journal={Archives of Environmental Contamination and Toxicology},
Abstract. Weathered Alaska North Slope crude oil (ANS 521) was subjected to biodegradation in vigorously stirred incubations for 14 days at 15 ± 1°C in 20‰ salinity sterilized seawater, amended with nutrients and inoculated with a hydrocarbon-degrading microorganism (EI2V) isolated from an oil-contaminated beach in Prince William Sound, Alaska. A total of 13.7 mg/L water-soluble neutral fraction (WSF) was recovered from the incubation of weathered ANS 521. Toxicity/teratogenicity tests were… 

Development and characterization of a cell line from Pacific herring, Clupea harengus pallasi, sensitive to both naphthalene cytotoxicity and infection by viral hemorrhagic septicemia virus

A cell line, PHL, has been successfully established from newly hatched herring larvae that are susceptible to infection by the North American strain of viral hemorrhagic septicemia (VHS) virus, and are sensitive to the cytotoxic effects of naphthalene, a common environmental contaminant.

Impacts to Pink Salmon Following the Exxon Valdez Oil Spill: Persistence, Toxicity, Sensitivity, and Controversy

L Laboratory tests verified that embryos are sensitive to long-term exposure to weathered oil in the low parts per billion range, which is important to the pink salmon fisheries of Prince William Sound and are also broadly applicable to toxicity and impact from nonpoint source pollution of urban estuaries.

Hydrocarbon composition and toxicity of sediments following the Exxon valdez oil spill in Prince William Sound, Alaska, USA

The low probability of exposure to toxic concentrations of weathered spill residues at the worst-case sites sampled in this study is consistent with the rapid overall recovery of shoreline biota observed in 1990 to 1991, and indicates the lack of potential for long-term toxic effects.

Synthesis of the toxicological impacts of the Exxon Valdez oil spill on Pacific herring (Clupea pallasi) in Prince William Sound, Alaska, U.S.A.

It is concluded that 25–32% of the embryos were damaged in PWS in 1989 and oil-induced mortality probably reduced recruitment of the 1989 year class into the fishery, but was impossible to quantify because recruitment was generally low in other Alaskan herring stocks.

Larval Pacific Herring (Clupea pallasi) Survival in Suspended Sediment

Pacific herring early life stages provide good model systems for studying effects of suspended sediments on estuarine organisms. To investigate effects on the herring larval stage, we used



Effect of Water Soluble Fraction of Prudhoe Bay Crude Oil on Embryonic Development of Pacific Herring

Abstract This project sought to simulate conditions of a crude oil spill to test the effects of low boiling point, water-soluble hydrocarbon components of Prudhoe Bay crude oil on developing Pacific

Microbial populations and hydrocarbon biodegradation potentials in fertilized shoreline sediments affected by the T/V Exxon Valdez oil spill

Elevated mineralization potentials, coupled with increased numbers of hydrocarbon degraders, indicated that natural hydrocarbon biodegradation was enhanced, but these microbiological measurements alone are not sufficient to determine in situ rates of crude oil biodegrading.

Ultrastructural effects of crude oil on early life stages of Pacific herring

Eggs of Pacific herring collected from Prince William Sound, Alaska were exposed to Prudhoe Bay crude oil for 4–144 hours, then returned to uncontaminated seawater for further development, finding many mitochondria in the body muscle of exposed organisms were swollen.

Selection of Nutrients to Enhance Biodegradation for the Remediation of Oil Spilled on Beaches1

ABSTRACT Laboratory studies were conducted to determine the fate of fertilizers proposed for application to the Alaska shoreline in support of the Alaskan Oil Spill EPA Bioremediation Project.

The Exxon Valdez oil spill: initial environmental impact assessment. Part 2

The March 24, 1989, grounding of the Exxon Valdez on Bligh Reef in Prince William Sound, Alaska, was unprecedented in scale. So too was Exxon's response to the oil spill and the subsequent shoreline

Exxon Valdez Oil Weathering Fate and Behavior: Model Predictions and Field Observations1

ABSTRACT Outdoor flow-through seawater wave tank studies and model predictions on the chemical and physical fate of Prudhoe Bay crude oil in subarctic waters are compared with field observations from

Stimulated biodegradation of oil slicks using oleophilic fertilizers.

A combination of paraffinized urea and octylphosphate was found to promote oil biodegradation both in laboratory experiments and in field trials to an extent that the practical application of this principle to oil cleanup appears feasible.

Effectiveness of bioremediation for the Exxon Valdez oil spill

A new interpretative technique used following the 1989 Exxon Valdez spill in Alaska shows that fertilizer applications significantly increased rates of oil biodegradation.

EPA's Alaska oil spill bioremediation project. Part 5

The significance and feasibility of the bioremediation of oil-contaminated environmental materials has increased and a more comprehensive understanding of the natural biodegradation potential of microbial communities in many different types of habitats is understood.

Sperm motility initiation factor is a minor component of the Pacific herring egg chorion

It was demonstrated that SMIF is present as a minor (4–7% of total chorion protein) component of the chorions of herring sperm motility initiation factor.