Pregnenolone sulfate augments NMDA receptor mediated increases in intracellular Ca2+ in cultured rat hippocampal neurons

@article{Irwin1992PregnenoloneSA,
  title={Pregnenolone sulfate augments NMDA receptor mediated increases in intracellular Ca2+ in cultured rat hippocampal neurons},
  author={Robert P. Irwin and Nicholas J. Maragakis and Michael A. Rogawski and Robert H. Purdy and David H. Farb and Steven M Paul},
  journal={Neuroscience Letters},
  year={1992},
  volume={141},
  pages={30-34}
}
The ability of the neuroactive steroid pregnenolone sulfate to alter N-methyl-D-aspartate (NMDA) receptor-mediated elevations in intracellular Ca2+ ([Ca2+]i) was studied in cultured fetal rat hippocampal neurons using microspectrofluorimetry and the Ca2+ sensitive indicator fura-2. Pregnenolone sulfate (5-250 microM) caused a concentration-dependent and reversible potentiation of the rise (up to approximately 800%) in [Ca2+]i induced by NMDA. In contrast, the steroid failed to alter basal… Expand
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