Pregnancies after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa.

@article{GilSalom1996PregnanciesAI,
  title={Pregnancies after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa.},
  author={Manuel Gil-Salom and Josep Ll Romero and Yolanda Bisbal M{\'i}nguez and C. Carrascoso Rubio and Maria Jos{\'e} de los Santos and Jose Alejandro Remohi and Antonio Pellicer},
  journal={Human reproduction},
  year={1996},
  volume={11 6},
  pages={
          1309-13
        }
}
In 25 patients (14 suffering from obstructive azoospermia, six from non-obstructive azoospermia, three from asthenoazoospermia and two from absence of ejaculation) spermatozoa were extracted from testicular biopsies. Intracytoplasmic sperm injection (ICSI) with fresh testicular spermatozoa was performed in 18 cases; spermatozoa in excess were cryopreserved in pills. No pregnancies were achieved. In the remaining seven patients, testicular spermatozoa were retrieved and cryopreserved during a… 
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136 Citations
Background Citations
26
Methods Citations
16
Results Citations
7

136 Citations

Citation Type

Citation Type

Intracytoplasmic Sperm Injection with Testicular Spermatozoa in Men with Azoospermia
TLDR
This study shows that the outcome of fresh and frozen–thawed testicular spermatozoa in ICSI is comparable, obstructive and nonobstructive etiologies perform the same, and that preincubation of testicular semen results in increased fertilization and pregnancy rates.
Intracytoplasmic sperm injection with cryopreserved testicular spermatozoa
Intracytoplasmic injection of fresh and cryopreserved testicular spermatozoa in patients with nonobstructive azoospermia--a comparative study.
Ongoing pregnancies after intracytoplasmic injection using cryopreserved testicular spermatozoa.
We report two clinical pregnancies occurring after intracytoplasmic sperm injection (ICSI) using cryopreserved spermatozoa obtained from testicular biopsy, made in two different infertility…
Ongoing pregnancies after intracytoplasmic injection using cryopreserved testicular spermatozoa
TLDR
Cryopreservation of surgically obtained spermatozoa obtained from testicular biopsy is reported, thuslimitingthenumber of necessary sperm epididymal collection procedures in patients suffering from obstructive or non-obstructiveazoospermia.
Testicular sperm extraction and cryopreservation in patients with non-obstructive azoospermia prior to ovarian stimulation for ICSI
TLDR
Cryo-thawed testicular sperm from NOA patients has the same fertilizing potential in ICSI programs, as the freshly retrieved sperm in those patients, TESE/cryopreservation has many advantages over Tese/ICSI in patients with NOA, so it should be considered as the first line in the therapy of those patients.
Testicular sperm extraction and intracytoplasmic sperm injection: a chance of fertility in nonobstructive azoospermia.
Intracytoplasmic sperm injection with motile and immotile frozen‐thawed testicular spermatozoa (the Hungarian experience)
TLDR
The data demonstrate that freezing of testicular spermatozoa opened new possibilities for the treatment of azoospermic men and the fertilization rate and frequency distribution of good‐quality embryos were lower in the case of immotile spermatozosa, and pregnancies were only achieved when motile spermutozoa had been used.
Testicular sperm retrieval and cryopreservation prior to initiating ovarian stimulation as the first line approach in patients with non-obstructive azoospermia.
TLDR
Criteria for predicting the presence of spermatozoa in the testicular tissue of patients with non-obstructive azoospermia are inadequate, it is suggested that TESE be performed prior to initiating ovarian stimulation.
Increased Fertilization Rates after In Vitro Culture of Frozen-Thawed Testicular Immotile Sperm in Nonobstructive Azoospermic Patients
TLDR
Fecundation rate can be significantly improved after in-vitro culture and sperm selection of frozen-thawed immotile testicular spermatozoa in patients with nonobstructive azoospermia.
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References

SHOWING 1-10 OF 20 REFERENCES
High fertilization and pregnancy rate after intracytoplasmic sperm injection with spermatozoa obtained from testicle biopsy.
TLDR
It appears that all cases of obstructive azoospermia can now be successfully treated and the few barely motile spermatozoa thus obtained can be used for ICSI.
Fertilization after intracytoplasmic sperm injection with cryopreserved testicular spermatozoa.
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TLDR
In this study, a total of 15 azoospermic patients suffering from testicular failure were treated with a combination of testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI), and three ongoing pregnancies out of 12 replacements were established.
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TLDR
This approach is offered as an alternative to the traditional scheme because it markedly eases the burden of partner scheduling on both the couple and the clinicians involved and assurance of the availability of male partner spermatozoa can be attained prior to beginning ovulation induction.
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TLDR
The results and rationale of using testicular and epididymal spermatozoa with intracytoplasmic sperm injection (ICSI) for severe cases of male infertility are reviewed and it is now clear that even with non-obstructive azoospermia, e.g. Sertoli-cell only, or maturation arrest, there are usually some small foci of spermatogenesis which allow TESE with ICSI to be carried out.
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TLDR
In seven patients who did not become pregnant following microsurgical epididymal sperm aspiration and intracytoplasmic sperm injection, it would appear mandatory to cryopreserve supernumerary spermatozoa during a MESA in order to avoid subsequent further scrotal surgery.
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High fertilization and implantation rates after intracytoplasmic sperm injection
TLDR
High pregnancy rates were noticed since 67 pregnancies were achieved, of which 53 were clinical, i.e. a total and clinical pregnancy rate of 44.7% and 35.3% per started cycle and 49.6% and 39.2% per embryo transfer.
Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles.
TLDR
Subzonal insemination and intracytoplasmic sperm injection were carried out in 300 treatment cycles in couples unable to be helped by conventional in-vitro fertilization treatment and normal fertilization rate was substantially higher after ICSI than after SUZI.
Using ejaculated, fresh, and frozen-thawed epididymal and testicular spermatozoa gives rise to comparable results after intracytoplasmic sperm injection.
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