Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte

  title={Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte},
  author={Gianpiero D. Palermo and Hubert Joris and Paul Devroey and Andr{\'e} C. Van Steirteghem},
  journal={The Lancet},
Intracytoplasmic sperm injection (ICSI) is a promising assisted-fertilisation technique that may benefit women who have not become pregnant by in-vitro fertilisation (IVF) or subzonal insemination (SUZI) of oocytes. We have used ICSI to treat couples with infertility because of severely impaired sperm characteristics, and in whom IVF and SUZI had failed. Direct injection of a single spermatozoon into the ooplasm was done in 47 metaphase-II oocytes: 38 oocytes remained intact after injection, 31… Expand

Paper Mentions

Interventional Clinical Trial
Over recent decades, the use of intracytoplasmic sperm injection (ICSI) has increased, even among patients without severe male factor infertility. Despite the increasing use, there is… Expand
Interventional Clinical Trial
The use of Intracytoplasmic sperm injection (ICSI) has increased in the last decades regardless of the cause of infertility. Despite the increased use there is no clear evidence… Expand
ConditionsInfertility, Female
Observational Clinical Trial
babies of known gender born out of 388 ICSI cycles were investigated for the gender ratio and then divided into three groups according to the sperm selection technique used before… Expand
ConditionsGender Ratio of ICSI Outcome
InterventionDevice, Other
Intracytoplasmic sperm injection
Different aspects of these novel assisted fertilization procedures are described: patient management, ovarian stimulation, semen evaluation and treatment, oocyte collection and preparation, SUZI and ICSI techniques, assessment of fertilization and embryo cleavage, outcome of embryo transfers and the evolution of the pregnancies. Expand
Triplet pregnancy and delivery after intracytoplasmic injection of round-headed spermatozoa.
Intracytoplasmic sperm injection (ICSI) of round-headed spermatozoa into mature oocyte resulted in normal fertilization, embryo development and pregnancy in a 28 year old female. The husband had aExpand
Intracytoplasmic sperm injection.
To evaluate the important issue of safety of the new technique of ICSi, a prospective follow-up study of children born after ICSI was carried out, compiling data on karyotypes, congenital malformations, growth parameters and developmental milestones. Expand
Application of intracytoplasmic sperm injection in assisted reproductive technologies.
A follow-up of the children born after ICSI treatment was conducted to address concerns as to the risk of transmitting genetic defects to the offspring. Expand
Factors affecting success with intracytoplasmic sperm injection.
ICSI has greatly improved the ability to use IVF for treating couples with a poor fertilization potential, and ICSI reinsemination was used with limited success to rescue failed fertilization cycles, although the implantation rate per embryo was poor. Expand
Assisted fertilization by subzonal insemination and intracytoplasmic sperm injection.
The results of 600 consecutive treatment cycles of subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) are described in couples with failed fertilization after standard IVF or insufficient spermatozoa in the ejaculate for IVF to indicate an increase in congenital malformations. Expand
Intracytoplasmic sperm injection: results from Norfolk, USA.
None of the sperm parameters of the original or processed semen sample were correlated with ICSI outcome, and female age and basal serum concentrations of follicle stimulating hormone (FSH) had a significant impact on implantation and pregnancy rates. Expand
Fertilization and pregnancies following intracytoplasmic injection of testicular spermatozoa
The ongoing pregnancies revealed no fetal abnormalities on ultrasound scanning and ICSI with testicular sperm performed in 16 couples performed with healthy men had ductal obstruction and failed previous attempts of epididymal sperm microaspiration. Expand
In vitro fertilization by intracytoplasmic sperm injection.
  • J. Tesarik, C. Mendoza
  • Biology, Medicine
  • BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1999
Intracytoplasmic sperm injection (ICSI) is the latest, and by far the most efficient, variant of micromanipulation-assisted fertilization, whereby a single spermatozoon is selected, aspirated into aExpand
Birth of a piglet derived from an oocyte fertilized by intracytoplasmic sperm injection (ICSI).
A live piglet derived from an embryo produced by intracytoplasmic sperm injection and carried to term was born to a mother that had received 32 embryos and was underweight, though healthy and lively. Expand


Induction of acrosome reaction in human spermatozoa used for subzonal insemination.
In three of the four ongoing pregnancies, prenatal diagnosis by amniocentesis indicated a normal karyotype, and there was no difference in the fertilization and cleavage rates between the two sperm treatment procedures. Expand
Microinjection of human oocytes: A technique for severe oligoasthenoteratozoospermia
Subzonal transfer may be a viable technique for patients with severe sperm problems and may help to determine outcome after microinjection with very poor quality sperm and after failed fertilization. Expand
Microinjection of human oocytes: a technique for severe oligoasthenoteratozoospermia *
Subzonal transfer may be a viable technique for patients with severe sperm problems after microinjection with very poor quality sperm and after failed fertilization. Expand
Cryopreservation of human embryos.
This chapter describes general cryobiological principles and the different methods for cryopreservation of supernumerary human embryos obtained after several procedures for medically assistedExpand
Enhancement of acrosome reaction and subzonal insemination of a single spermatozoon in mouse eggs
The mean percentages of acrosome‐free spermatozoa obtained by these various methods and assessed on the basis of both Bryan's stain and immunolocalization by FITC‐labeled monoclonal antibodies increased by steps from 36% to 67%, 73%, 86%, and 92%. Expand
Micromanipulation of gametes for in vitro assisted fertilization
  • A. Iritani
  • Biology, Medicine
  • Molecular reproduction and development
  • 1991