Preferential nuclear accumulation of JAK2V617F in CD34+ but not in granulocytic, megakaryocytic, or erythroid cells of patients with Philadelphia-negative myeloproliferative neoplasia.

@article{Rinaldi2010PreferentialNA,
  title={Preferential nuclear accumulation of JAK2V617F in CD34+ but not in granulocytic, megakaryocytic, or erythroid cells of patients with Philadelphia-negative myeloproliferative neoplasia.},
  author={Ciro Roberto Rinaldi and Paola Rinaldi and Adele Alagia and Marica Gemei and Nicola Esposito and Fabio Formiggini and Vincenzo Martinelli and Vitalyi Senyuk and Giuseppina Nucifora and Fabrizio Pane},
  journal={Blood},
  year={2010},
  volume={116 26},
  pages={6023-6}
}
Recently, Dawson et al identified a previously unrecognized nuclear role of JAK2 in the phosphorylation of histone H3 in hematopoietic cell lines. We searched nuclear JAK2 in total bone marrow (BM) cells and in 4 sorted BM cell populations (CD34(+), CD15(+), CD41(+), and CD71(+)) of 10 myeloproliferative neoplasia (MPN) patients with JAK2V617F mutation and 5 patients with wild-type JAK2 MPN. Confocal immunofluorescent images and Western blot analyses of nuclear and cytoplasmic fractions found… CONTINUE READING