Preferential cleavage of plasmid-based R-loops and D-loops by Drosophila topoisomerase IIIbeta.

@article{WilsonSali2002PreferentialCO,
  title={Preferential cleavage of plasmid-based R-loops and D-loops by Drosophila topoisomerase IIIbeta.},
  author={Tina Wilson-Sali and Tao-shih Hsieh},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99 12},
  pages={7974-9}
}
The topoisomerase (topo) III enzymes are found in organisms ranging from bacteria to humans, yet the precise cellular function of these enzymes remains to be determined. We previously found that Drosophila topo IIIbeta can relax plasmid DNA only if the DNA is first hypernegatively supercoiled. To investigate the possibility that topo IIIbeta requires a single-stranded region for its relaxation activity, we formed R-loops and D-loops in plasmids. In addition to containing a single-stranded… CONTINUE READING
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