Preferential V beta gene usage and lack of junctional sequence conservation among human T cell receptors specific for a tetanus toxin- derived peptide: evidence for a dominant role of a germline-encoded V region in antigen/major histocompatibility complex recognition

@article{Boitel1992PreferentialVB,
  title={Preferential V beta gene usage and lack of junctional sequence conservation among human T cell receptors specific for a tetanus toxin- derived peptide: evidence for a dominant role of a germline-encoded V region in antigen/major histocompatibility complex recognition},
  author={Brigitte Boitel and Myriam Ermonval and Paola Panina-Bordignon and Roy A. Mariuzza and Antonio Lanzavecchia and Oreste Acuto},
  journal={The Journal of Experimental Medicine},
  year={1992},
  volume={175},
  pages={765 - 777}
}
To investigate the structural and genetic basis of the T cell response to defined peptide/major histocompatibility (MHC) class II complexes in humans, we established a large panel of T cell clones (61) from donors of different HLA-DR haplotypes and reactive with a tetanus toxin-derived peptide (tt830-844) recognized in association with most DR molecules (universal peptide). By using a bacterial enterotoxin-based proliferation assay and cDNA sequencing, we found preferential use of a particular… CONTINUE READING