Preferential Activity of Wild‐type and Mutant Tumor Necrosis Factor‐α against Tumor‐derived Endothelial‐like Cells

  title={Preferential Activity of Wild‐type and Mutant Tumor Necrosis Factor‐$\alpha$ against Tumor‐derived Endothelial‐like Cells},
  author={Kensei Kuroda and Keizo Miyata and Yasuo Tsutsumi and Shin-ichi Tsunoda and Koji Nishimura and Yachiyo Mitsuishi and Shinsaku Nakagawa and and Tadanori Mayumi},
  journal={Japanese Journal of Cancer Research : Gann},
  pages={59 - 67}
Tumor‐derived endothelial‐like cells (tEC) were prepared by culturing human umbilical vein endothelial cells (HUVEC) in the presence of HT1080 human fibrosarcoma‐conditioned medium. tEC showed higher permeability and less cell‐adhesion activity than normal HUVEC (nEC). Tumor necrosis factor‐α (TNF) is known to have tumor‐vasculature disrupting activity. tEC showed higher cytotoxicity to recombinant human TNF (rhTNF) than nEC, and was not observed using HUVEC cultured with WI38 human diploid… 

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