Preference for IgG mAb binding insulin in solution or on surfaces is related to immunoglobulin variable region structures.

@article{Tikhomirov1997PreferenceFI,
  title={Preference for IgG mAb binding insulin in solution or on surfaces is related to immunoglobulin variable region structures.},
  author={Oleg Y Tikhomirov and James W. Thomas},
  journal={Journal of autoimmunity},
  year={1997},
  volume={10 6},
  pages={541-9}
}
Studies on insulin autoantibodies often show a lack of correlation between enzyme-linked immunosorbent assays (ELISAs) and fluid phase radioimmunoassays (RIAs). Similarly, a set of IgG anti-insulin monoclonal antibodies (mAb) from BALB/c mice are found to differ in their binding in ELISAs and RIAs. To understand the structural basis for differential insulin binding, soluble and complexed biotinylated insulin is used to confirm binding properties independent of insulin-coated plastic and… CONTINUE READING