Predominant role of alpha 4-integrins for distinct steps of lymphoma metastasis.


To analyze the role of alpha4-integrins in lymphoma metastasis, sublines of the T-cell lymphoma LB were generated by retrovirus-mediated gene transfer that differ exclusively in the expression of alpha4-integrins. Using LB-alpha4 and control LB-NTK cells, we demonstrate that expression of alpha4-integrins strongly suppresses metastasis formation of LB lymphoma cells in secondary lymphoid organs such as spleen, mesenteric and peripheral lymph nodes, or Peyer's patches after i.v. injection into syngeneic BALB/c mice. Moreover, alpha4-integrin expression inhibited development of metastatic tumors in liver, lung, and kidney. Expansion of LB lymphoma cells in bone marrow was not affected by alpha4-integrin expression. In vivo migration assays using 51Cr-labeled lymphoma cells demonstrated that low-metastatic LB-alpha4 cells accumulated with the same efficiency as high-metastatic LB-NTK cells in all target organs examined and were even enriched in mucosal lymphoid organs. Collectively, these results indicate that alpha4-integrins inhibit metastasis formation of lymphoma cells at a stage subsequent to the invasion of target organs.


Citations per Year

82 Citations

Semantic Scholar estimates that this publication has 82 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Gosslar1996PredominantRO, title={Predominant role of alpha 4-integrins for distinct steps of lymphoma metastasis.}, author={U. Gosslar and P. Jonas and Arne Luz and A Lifka and David Naor and Alf Hamann and Bernhard Holzmann}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={1996}, volume={93 10}, pages={4821-6} }