Predisposition to neoplastic transformation caused by gene replacement of H-ras1.

@article{Finney1993PredispositionTN,
  title={Predisposition to neoplastic transformation caused by gene replacement of H-ras1.},
  author={R E Finney and J. Michael Bishop},
  journal={Science},
  year={1993},
  volume={260 5113},
  pages={1524-7}
}
Homologous recombination was used to introduce a nominally transforming mutation into an endogenous H-ras1 gene in Rat1 fibroblasts. Although both the mutant and the remaining normal allele were expressed equally, the heterozygous cells were not neoplastically transformed. Instead, spontaneously transformed cells arose from the heterozygotes at a low frequency, and the majority of these cells had amplified the mutant allele. Thus, the activated H-ras1 allele was not by itself dominant over the… CONTINUE READING

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