Prediction of missed proteolytic cleavages for the selection of surrogate peptides for quantitative proteomics.

@article{Lawless2012PredictionOM,
  title={Prediction of missed proteolytic cleavages for the selection of surrogate peptides for quantitative proteomics.},
  author={Craig Lawless and Simon J. Hubbard},
  journal={Omics : a journal of integrative biology},
  year={2012},
  volume={16 9},
  pages={449-56}
}
Quantitative proteomics experiments are usually performed using proteolytic peptides as surrogates for their parent proteins, inferring protein amounts from peptide-level quantitation. This process is frequently dependent on complete digestion of the parent protein to its limit peptides so that their signal is truly representative. Unfortunately… CONTINUE READING