Prediction of effective RNA interference targets and pathway-related genes in lepidopteran insects by RNA sequencing analysis.


When using RNA interference (RNAi) to study gene functions in Lepidoptera insects, we discovered that some genes could not be suppressed; instead, their expression levels could be up-regulated by double-stranded RNA (dsRNA). To predict which genes could be easily silenced, we treated the Asian corn borer (Ostrinia furnacalis) with dsGFP (green fluorescent… (More)
DOI: 10.1111/1744-7917.12437